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David A. Maclean, PH.D.

maclean_david.jpg Northern Ontario School of Medicine
Lakehead University Campus
935 Ramsey Lake Road
Sudbury ON Canada P3E 2C6
Phone:  (705) 662-7240
Fax:  (705) 675-4858
E-mail: david.maclean@nosm.ca
Professor of Physiology, Division of Medical Sciences, Northern Ontario School of Medicine
Cross Appointment, Department of Biology Laurentian University

Education/Training

 2001-2004

Associate Professor, Exercise Science
School of Exercise, Leisure and Sport, Kent State University, Kent, OH.

1999-2001

Director, Microdialysis Core Laboratory, General Clinical Research Center (GCRC)
The Pennsylvania State University College of Medicine
The Milton S. Hershey Medical Center, Hershey, PA

 1998-2001

Assistant Professor of Medicine and Cellular and Molecular Physiology
The Pennsylvania State University College of Medicine
The Milton S. Hershey Medical Center, Hershey, PA

1996-1997

Post-doctoral fellow, Division of Cardiology
The Pennsylvania State University College of Medicine
The Milton S. Hershey Medical Center, Hershey, PA

1994-1996

Post-doctoral fellow, Copenhagen Muscle Research Centre.
Rigshospitalet, section 7652, Copenhagen N, Denmark

1993

Ph.D. Biophysics
University of Guelph

1989

M.Sc. Human Biology
University of Guelph

1987

B.Sc. Human Kinetics
University of Guelph

Research Investigations

My research is focused on the better understanding of cardiovascular physiology as it pertains to the regulation of blood flow under conditions of hypoxia, vascular insufficiency and end stage disease states such as heart disease. I employ both human and animal models to examine these questions including muscle tissue sampling arterio-venous blood collections. Although these have methods have provide valuable information regarding the vascular mechanisms associated with blood flow, they do not sample all the physiological compartments. More specifically, the interstitial space. Therefore, I utilize a very unique procedure, the microdialysis technique to directly sample and quantitate compounds in the interstitial space, hence investigating physiological parameters directly at the tissue level. This is a very powerful technique that is only performed in a handful of laboratories and represents a considerable advance in our ability to understand the mechanisms associated with cardiovascular regulation.

An associated area of research is protein and amino acid metabolism under both normal and abnormal physiological conditions. For example, I examine the incorporation of amino acids into muscle protein during anabolic conditions such as exercise as well as during end stage disease states such as heart failure. These studies will hopefully provide new insights into the regulation and potential countermeasures associated with heath and disease.

Selected Publications

Vissing J, MacLean DA, Vissing SF, Sander M, Saltin B, Haller RG. The exercise metaborereflex is maintained in the absence of muscle acidosis: insights from muscle microdialysis in humans with McArdle’s disease. J Physiol 537.2: 641-649, 2001.

Anthony CJ, Reiter AK, Anthony TG, Crozier SJ, Lang CH, MacLean DA, Kimball SR, Jefferson LS. Orally administered leucine enhances protein synthesis in skeletal muscle of diabetic rats in the absence of increases in 4E-BP1 or S6K1 phosphorylation. Diabetes 51: 928-936, 2002.

Anthony CJ, Lang CH, Crozier SJ, Anthony TG, MacLean DA, Kimball SR, Jefferson LS. Contribution of insulin to the translational control of protein synthesis in skeletal muscle by leucine. Am J Physiol 282: E1092-E1101, 2002.

Calbet JAL, MacLean DA. Plasma glucagon and insulin responses depend on the rate of appearance of amino acids after ingestion of different protein solutions in humans. J Nutr 132: 2174-2182, 2002.

Lang CH, Frost RA, NaimAC, MacLean DA, Vary TC. TNF-alpha impairs heart and skeletal muscle protein synthesis by altering translation initiation. Am J Physiol 282: E336-347, 2002.

Khan MH, Sinoway LI, MacLean DA. The effects of graded LBNP on MSNA and interstitial norepinephrine. Am J Physiol 283: H2038-H2044, 2002.

Proctor DN, Newcomer SC, Koch DW, Le KU, MacLean DA, Leuenberger UA. Leg blood flow during submaximal cycle ergometry is not reduced in healthy older normally active men. J Appl Physiol 94: 1859-1869, 2003.

Samii S, Khan M, MacLean DA, King N, Herr MD, Sinoway LI. Muscle interstitial calcium during head-up tilt in humans. Circulation 109: 215-219, 2004.

Chinkes D, MacLean DA, Gore D, Wolfe RR. In Vivo Muscle Amino Acid Transport Involves Two Distinct Processes. Am J Physiol 287: E136-141, 2004.

Bolster DR, Pikosky MA, Bennett BT, Maresh CM, Tipton KD, MacLean DA, Rodriguez NR. Dietary Protein Intake Impacts Human Skeletal Muscle Protein Fractional Synthetic Rates Following Endurance Exercise.Am J Physiol. (In Press).

Rush JWE, Green, HG, MacLean DA, Code LM. Oxidative Stress Determinants and Nitric Oxide Synthase in Skeletal Muscle of Rats with Chronic Heart Failure. Acta Physiol Scand (In Press)